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Устройство OLYMPUS LV200. Инструкция на английском языке

Руководство пользователя OLYMPUS LV200. Основные функции, характеристики и условия эксплуатации изложены на 2 страницах документа в pdf формате.

Информация

Раздел
Оборудование
Категория
Телескопы / микроскопы / бинокли
Тип устройства
Устройство
Производитель (бренд)
OLYMPUS
Модель
OLYMPUS LV200
Еще инструкции
Телескопы / микроскопы / бинокли OLYMPUS, Устройство OLYMPUS
Язык инструкции
английский
Дата создания
19 Сентября 2018 г.
Просмотры
81 просмотр
Количество страниц
2
Формат файла
pdf
Размер файла
32.94 Кб
Название файла
olympus_manual_lv200.pdf

Фото

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  • LV200 Installation:
    1)  Place the system in a dark room if possible or avoid direct strong light!
    2)  Install system table.
    3)  Place LV200 onto table right above the prepared whole.
    4)  Don’t place the PC, controllers onto the table (vibrations)
    5)  Connect transmitted light source
    6)  If you want to use filter wheels in cellM connect UCB with PC
    7)  The filter wheels are marked at the cable with FW1 and FW2. Connect them in the vice
    versa
    order into UCB ( FW1 in 2. and FW2 in 1.)  !
    Choose “IX/51/71” in OBS config
    Choose “Contrast inserts” for upper filter wheel including blockers
    Choose “Filter Cubes” for lower filter wheel (empty in standard configuration)
    8)  Connect cables for temperature control (connection cable at the back of LV200)
    9)  Screw in the tube lens carefully from the bottom
    10) Camera installation:
    a)  Fix the c-mount adaptor onto the camera
    b)  Remove the small black metal part at the front to get access to the mounting
    screw.
    c)  Use the long hex wrenge 3 (small whole at the front) to fix the camera. Caution
    the camera may fall down !!
    11) The camera is used without a trigger cable to avoid heating of the chip
    12) To change the objectives you have to loose the screws for the incubation chamber.
    During a
    demo please leave this screws loose, to have the possibility of changing the objective
    13) You can only use 35 mm Petri dishes (and with some limitations also slides). Best glass
    bottom Petri dishes
    Important points for Software:  only for cellM  (until now not possible with Andor
    iKonM)
    1)  The integration times will be much higher as in fluorescence may be 100 000 ms or
    even 1 000 000 ms.
    2)  The 100 s limit of integration time is solved from version 3.1
    3)  In the standard configuration there are light blockers in every second position of the
    filter wheel. In bioluminescence mode use them to prevent light passing the light
    guide. Turn off light source during long experiments. Lowest energy level and almost
    closed shutter at the light source gives
    enough light.
    4)  Bright field. Change light blockers by hand switch or PC after you have decreased the
    integration time lower than 50 ms.
    5) Acquisition using experiment manager:
    don’t use trigger cable. The chip will get too warm.
    only use “simple acquisition” instead of image type button
    use “non synchronous” mode
    ad a time loop around the acquisition (is needed for nonsynchronous mode)
  • change the gain in live mode to the needed value.
    Experiments:
    1)  The customer has to know the difference between fluorescence and luminescence.
    Signals are much weaker (factor 1000 to 1 000 000 !!!)
    2)  Highest intensity with low magnifications!
    3)  Due to 0.2 tube lens magnifications of a 20x lens means 4x in reality!
    4)  Use always glass bottom dishes for higher magnifications.
    5)  The sample has to be completely stable. During the long integration times movements
    or vibrations will lead to a complete loss of the signal.
    6)  Please tell customer not to open light source or changing filter wheel (blocker) during
    long exposure.
    This can damage the camera. Keep the intensity of light source at the minimal position.
    7)  Some luminescent sources need oxygen. They will not work under a cover slip!
    8)   Focus:  The focus of transmission and luminescence is not the same!
    9)   Focus with transmission (change position of upper filter wheel using hand switch
    until you see the light.
    10)  Reduce light to a minimum (lowest aperture and energy) !!
    11)  The luminescence focus is just between the transmission focus  “ black granules in
    cells”  to  “glass like appearance”
    12)  In most cases you will not see live luminescence!
    13)  For luminescence switch upper filter position to the next position which includes
    blocker or use
    PC control (condenser)
    The expectations of the customer will be sometimes unrealistic. They will compare it with
    fluorescence
    and confocal without thinking. Always ask them if they ever have seen a luminescence image!

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