Устройство OLYMPUS CellSens 1.6. Инструкция на английском языке

User Manual
cellSens
LIFE SCIENCE IMAGING SOFTWARE

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Version  510_UMA_cellSens16_Han_en_00
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Contents
1.  About the documentation for your software................................................... 4
2.  User interface .................................................................................................... 5
2.1.Overview - User interface......................................................................................................... 5
2.2.Overview - Layouts................................................................................................................... 6
2.3.Document group....................................................................................................................... 7
2.4.Tool Windows........................................................................................................................... 8
2.5.Working with documents .......................................................................................................... 9
3.  Configuring the system .................................................................................. 12
4.  Acquiring single images................................................................................. 14
5.  Acquiring image series................................................................................... 18
5.1.Time stack .............................................................................................................................. 18
5.2.Time Lapse / Movie................................................................................................................ 20
5.3.Acquiring movies and time stacks.......................................................................................... 21
5.4.Z-stack.................................................................................................................................... 24
5.5.Acquiring a Z-stack................................................................................................................. 26
6.  Acquiring fluorescence images ..................................................................... 28
6.1.Multi-channel image ............................................................................................................... 28
6.2.Before and after you've acquired a fluorescence image........................................................ 31
6.3.Defining observation methods for the fluorescence acquisition............................................. 33
6.4.Acquiring and combining fluorescence images...................................................................... 38
6.5.Acquiring multi-channel fluorescence images........................................................................ 44
7.  Creating stitched images................................................................................ 50
8.  Processing images.......................................................................................... 57
8.1.Fluorescence Unmixing.......................................................................................................... 58
9.  Measuring images ........................................................................................... 63
9.1.Measuring images.................................................................................................................. 65
9.2.Measuring intensity profiles.................................................................................................... 71
9.3.Measuring the colocalization.................................................................................................. 74